Journal: Epigenetics
Article Title: HIV-1 Tat and cocaine impact mitochondrial epigenetics: effects on DNA methylation
doi: 10.1080/15592294.2020.1834919
Figure Lengend Snippet: Mitochondrial methylation is shown with reference to the human mitochondrial genome. A) Sixty-four bisulphite PCR primers were designed using Qiagen ADSW to investigate 326 CpG sites in the mitochondrial genome. mtDNA methylation was analysed by TNGBS of the mitochondrial genome in primary astrocytes. B) The mtDNA methylation shown at different CpG sites is representative of three independent experiments, and the results were aligned to the human mitochondrial genome (NCBI reference sequence: NC_012920.1, length: 16,569 bp). *P < 0.05
Article Snippet: A) Sixty-four bisulphite PCR primers were designed using Qiagen ADSW to investigate 326 CpG sites in the mitochondrial genome. mtDNA methylation was analysed by TNGBS of the mitochondrial genome in primary astrocytes.
Techniques: Methylation, Sequencing